Expression Kinetics and Mapping of the Thymidine Kinase Transcript and an Immediate-Early Transcript from Channel Catfish Virus
نویسندگان
چکیده
منابع مشابه
Expression kinetics and mapping of the thymidine kinase transcript and an immediate-early transcript from channel catfish virus.
Three transcripts from the terminal repeat of the channel catfish virus (CCV; also known as ictalurid herpesvirus 1) genome were mapped by S1 nuclease and primer extension analyses as well as by cDNA sequencing. These transcripts, TR3, TR5/6, and TR6, are encoded by open reading frame (ORF) 3, ORFs 5 and 6, and ORF 6, respectively, and correspond to those previously identified by sequence analy...
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15 صفحه اولIdentification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene.
A HeLa cell transient-expression assay system was used to determine if isolated immediate early (alpha) genes from herpes simplex virus (HSV) could transcriptionally activate (transactivate) the type 1 (HSV-1) thymidine kinase (TK) gene [an early (beta) gene]. Cells transfected with the TK gene alone transcribed very low levels of TK RNA. Cells cotransfected with plasmids bearing the sequences ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1998
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.72.5.3900-3906.1998